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ISBN 10: 0080497143
ISBN 13: 9780080497143
Author: Carl Wu, David Allis
DNA in the nucleus of plant and animal cells is stored in the form of chromatin. Chromatin and the Chromatin remodelling enzymes play an important role in gene transcription.
Section I: Biophysics, Structural Biology, and Enzymology of Chromatin Proteins
Chapter 1. Fluorescence Anisotropy Assays for Analysis of ISWI-DNA and ISWI-Nucleosome Interactions
Chapter 2. Biophysical Analysis of Specific Genomic Loci Assembled as Chromatin In Vivo
Chapter 3. Visualization and 3D Structure Determination of Defined Sequence Chromatin and Chromatin
Chapter 4. Electron Microscopic Analysis of the RSC Chromatin Remodeling Complex
Chapter 5. Use of Optical Trapping Techniques to Study Single-Nucleosome Dynamics
Chapter 6. Single-Molecule Analysis of Chromatin
Chapter 7. Biochemical and Structural Characterization of Recombinant Histone Acetyltransferase Prot
Chapter 8. Use of Nuclear Magnetic Resonance Spectroscopy to Study Structure-Function of Bromodomain
Chapter 9. Assays for the Determination of Structure and Dynamics of the Interaction of the Chromodo
Chapter 10. Expression, Purification, and Biophysical Studies of Chromodomain Proteins
Chapter 11. Quantitative Assays for Characterization of the Sir2 Family of NAD+-Dependent Deacetylas
Chapter 12. Selective HAT Inhibitors as Mechanistic Tools for Protein Acetylation
Chapter 13. Histone Deacetylase Inhibitors: Assays to Assess Effectiveness In Vitro and In Vivo
Section II: Immunochemical Assays of Chromatin Functions
Chapter 14. Immunochemical Analysis of Chromatin
Chapter 15. Generation and Characterization of Antibodies Directed Against Di-Modified Histones, and
Chapter 16. Generation and Characterization of Methyl-Lysine Histone Antibodies
Chapter 17. Tips in Analyzing Antibodies Directed Against Specific Histone Tail Modifications
Chapter 18. Histone Methylation: Recognizing the Methyl Mark
Chapter 19. Analysis of Genome-Wide Histone Acetylation State and Enzyme Binding Using DNA Microarra
Chapter 20. Use of Chromatin Immunoprecipitation Assays in Genome-Wide Location Analysis of Mammalia
Chapter 21. High-Throughput Screening of Chromatin Immunoprecipitates Using CpG-Island Microarrays
Chapter 22. Chromatin Immunoprecipitation in the Analysis of Large Chromatin Domains Across Murine A
Chapter 23. The Use of Chromatin Immunoprecipitation Assays in Genome-Wide Analyses of Histone Modif
Chapter 24. Sequential Chromatin Immunoprecipitation from Animal Tissues
Chapter 25. Immunofluorescent Staining of Polytene Chromosomes: Exploiting Genetic Tools
Chapter 26. Indirect Immunofluorescent Labeling of Drosophila Polytene Chromosomes: Visualizing Prot
Chapter 27. X-Chromosome Inactivation in Mouse Embryonic Stem Cells: Analysis of Histone Modificatio
Chapter 28. X Inactivation in Mouse ES Cells: Histone Modifications and FISH
Author Index
Subject Index
chromatin remodeling systems
chromatin remodeling and histone modifications
chromatin remodeling examples
chromatin remodeling review
a chromatin
Tags: Carl Wu, David Allis, Chromatin Remodeling, Part B
