Dissecting Microbial Communities With Singlecell Transcriptome Analysis Andrew W Pountain by Andrew W. Pountain, Itai Yanai 10.1126/SCIENCE.ADP625 instant download after payment.

BACKGROUND: Microbes rarely exist as isolated cells but instead treatment, and mobile genetic element activity. Recently, similar form dynamic, interactive communities that play critical roles in methods have also been applied to more challenging communities, human development, metabolism, and immunity. Although such as those colonizing the mammalian gut. These developments investigations of these microbiomes have largely focused on these have enabled deeper exploration of functional diversification within interactions at the level of species or higher microbial taxa, even microbial species, and in turn how host-derived environmental cues cells of a single species can differ substantially in their gene content, shape microbial states.physiology, and environment. This intraspecies heterogeneity may lead to functional diversification, whereby cellular subpopulations
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OUTLOOK: Single-cell transcriptomics are poised to redefine how we play different functional roles and influence survival in the face of understand microbiome function and dynamics. Beyond studying external threats such as antibiotic stress. Single-cell transcriptomics the divisions of labor that support community-level behavior in has transformed our understanding of cellular diversity in simple biofilms, these technologies now allow us to explore these mammalian systems, yet microbes are far smaller than mammalian interactions across natural, multispecies ecosystems. Phenotypic cells and are protected by cell walls.