Human Retrovirus Protocols Virology and Molecular Biology Methods in Molecular Biology 304 1st Edition by Tuofu Zhu ISBN 1617375993 978-1617375996 by Tuofu Zhu 9781592599073, 1592599079 instant download after payment.

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Product details:

ISBN 10: 1617375993

ISBN 13: 978-1617375996 

Author: Tuofu Zhu

Table of contents:

1. Methods for Virus Isolation and Detection

2. Enhanced Culture Assay for Detection and Quantitation of Latently Infected, Resting CD4+ T-Cells Carrying Replication-Competent Virus in HIV-1-Infected Individuals

3. Isolation, Propagation, and Titration of Human Immunodeficiency Virus Type 1 From Peripheral Blood of Infected Individuals

4. Isolation of Human Immunodeficiency Virus Type 1 From Peripheral Blood Monocytes

5. Isolation, Propagation, and HIV-1 Infection of Monocyte-Derived Macrophages and Recovery of Virus From Brain and Cerebrospinal Fluid

6. Isolation and HIV-1 Infection of Primary Human Microglia From Fetal and Adult Tissue

7. Isolation of Human Immunodeficiency Virus Type 1 From Semen and Vaginal Fluids

8. Isolation and Quantification of HIV From Lymph Nodes

9. Isolation of Human Immunodeficiency Virus Type 2 Biological Clones From Peripheral Blood Lymphocytes

10. Isolation and Confirmation of Human T-Cell Leukemia Virus Type 2 From Peripheral Blood Mononuclear Cells

11. Isolation of Foamy Viruses From Peripheral Blood Lymphocytes

12. Alu-LTR Real-Time Nested PCR Assay for Quantifying Integrated HIV-1 DNA

13. Quantification of HFV-Integrated DNA in Human Cells by Alu-LTR Real-Time PCR

14. Detection of HIV-1 Provirus and RNA by In Situ Amplification

15. Detection of HTLV-1 Gene on Cytologic Smear Slides

16. Detection of HIV-2 by PCR

17. Quantitation of HIV-1 Viral RNA in Blood Plasma and Genital Secretions

18. Quantification of Proviral DNA Load of Human Immunodeficiency Virus Type 2 Subtypes A and B Using Real-Time PCR

19. Plasma RNA Viral Load in HIV-1 Group O Infection by Real-Time PCR

20. A New Combined HIV p24 Antigen and Anti-HIV-1/2/O Screening Assay

21. A New Automated Fourth-Generation HIV Screening Assay With Sensitive Antigen Detection Module and High Specificity

22. Detection of Antiviral Antibodies Using Enzyme-Linked Immunosorbent Assay

23. Plaque-Reduction Assays for Human and Simian Immunodeficiency Virus Neutralization

24. Detection of Drug-Resistant HIV-1 Strains

25. Molecular Biology Methods

26. Determination of Cell Tropism of HIV-1

27. Determination of Co-Receptor Usage of HIV-1

28. Quantitative Evaluation of HIV and SIV Co-Receptor Use With GHOST(3) Cell Assay

29. Phenotypic Characterization of Blood Monocytes From HIV-Infected Individuals

30. Methods to Determine HIV-1 Ex Vivo Fitness

31. A Yeast Recombination-Based Cloning System to Produce Chimeric HIV-1 Viruses and Express HIV-1 Genes

32. PCR Amplification, Cloning, and Construction of HIV-1 Infectious Molecular Clones From Virtually Full-Length HIV-1 Genomes

33. Amplification and Cloning of Near Full-Length HIV-2 Genomes

34. Growth and Manipulation of a Human T-Cell Leukemia Virus Type 2 Full-Length Molecular Clone

35. Construction and Analysis of Genomic, Full-Length Infectious Foamy Virus DNA Clones

36. Molecular Characterization of Proteolytic Processing of the Gag Proteins of Human Spumaretrovirus

37. Assessing the Relative Efficacy of Antiretroviral Activity of Different Drugs on Macrophages

38. Gene Expression Profiling of HIV-1 Infection Using cDNA Microarrays

39. Determination of DC-SIGN and DC-SIGNR Repeat Region Variations

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Tags: Tuofu Zhu, Human Retrovirus, Protocols, Virology, Molecular Biology, Methods in Molecular Biology, Volume 304