Maternal Gut Microbiota Influence Stem Cell Function In Offspring Haiyue Dang by Haiyue Dang, Panpan Feng, Shuning Zhang, Lihua Peng, Shuli Xing, Yuchen Li, Xiang Wen, Liqiang Zhou, Shyamal Goswami, Mingbing Xiao, Nick Barker, Philippe Sansonetti, Parag Kundu 10.1016/J.STEM.2024.10.003 instant download after payment.

The maternal microbiome influences child health. However, its impact on a given offspring’s stem cells, whichregulate development, remains poorly understood. To investigate the role of the maternal microbiome in conditioning the offspring’s stem cells, we manipulated maternal microbiota using Akkermansia muciniphila.Different maternal microbiomes had distinct effects on proliferation and differentiation of neuronal and intestinal stem cells in the offspring, influencing their developmental trajectory, physiology, and long-term health.Transplantation of altered maternal microbiota into germ-free mice transmitted these stem cell phenotypesto the recipients’ offspring. The progeny of germ-free mice selectively colonized with Akkermansia did notdisplay these stem cell traits, emphasizing the importance of microbiome diversity. Metabolically more activematernal microbiomes enriched the levels of circulating short-chain fatty acids (SCFAs) and amino acids,leaving distinct transcriptomic imprints on the mTOR pathway of offsprings’ stem cells. Blocking mTORsignaling during pregnancy eliminated the maternal-microbiome-mediated effects on stem cells. Theseresults suggest a fundamental role of the maternal microbiome in programming offsprings’ stem cells andrepresent a promising target for interventions.