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5.0
108 reviewsSpatial transcriptomics enables gene expression mapping within tissues but is often limited by imaging constraints. We present an imaging-free approach that reconstructs spatial barcode locations using molecular difusion and dimensionality reduction. Validated against ground truth imaging, our method achieves high fdelity and scales to centimeter-sized tissues. This approach enhances spatial transcriptomics’ accessibility and throughput, enabling large-scale studies without specialized imaging equipment.
