Singlecell Atlas Of Endothelial And Mural Cells Across Primary And Metastatic Brain Tumors Leire Bejarano by Leire Bejarano, Joao Lourenco, Annamaria Kauzlaric, ..., Roy T. Daniel, Monika E. Hegi, Johanna A. Joyce 101016/JIMMUNI202502022 instant download after payment.

SUMMARYCentral nervous system (CNS) malignancies include primary tumors, such as gliomas, and brain metastases(BrMs) originating from diverse extracranial cancers. The blood-brain barrier (BBB) is a key structural component of both primary and metastatic brain cancers. Here, we comprehensively analyzed the two major BBBcell types, endothelial and mural cells, across non-tumor brain tissue, isocitrate dehydrogenase (IDH) mutant(IDH mut) low-grade gliomas, IDH wild-type (IDH WT) high-grade glioblastomas (GBMs), and BrMs fromvarious primary tumors. Bulk and single-cell RNA sequencing, integrated with spatial analyses, revealedthat GBMs, but not low-grade gliomas, exhibit significant alterations in the tumor vasculature, includingthe emergence of diverse pathological vascular cell subtypes. However, these alterations are less pronounced in GBMs than in BrMs. Notably, the BrM vasculature shows higher permeability and more extensiveinteractions with distinct immune cell populations. This vascular atlas presents a resource toward understanding of tumor-specific vascular features in the brain, providing a foundation for developing vascularand immune-targeting therapies.