Alternative Premrna Splicing Theory And Protocols Stefan Stamm by Stefan Stamm, Chris Smith, And Reinhard Luhrmann (editors) 9783527326068, 9783527636778, 3527326065, 3527636773 instant download after payment.

The book consists of two major parts: The first one provides a brief theoretical
introduction that gives a short overview of alternative splicing and cites key papers in the field for more in-depth information. The second part is a collection of experimental protocols that are used in the field of alternative splicing.
Contents
1 Splicing in the RNA World
2 RNPs, Small RNAs, and miRNAs
3 RNA Elements Involved in Splicing
4 A Structural Biology Perspective of Proteins Involved in Splicing Regulation
5 The Spliceosome in Constitutive Splicing
6 The Use of Saccharomyces cerevisiae to Study the Mechanism of pre-mRNA Splicing
7 Challenges in Plant Alternative Splicing
8 Alternative Splice Site Selection
9 Integration of Splicing with Nuclear and Cellular Events
10 Splicing and Disease
11 From Bedside to Bench: How to Analyze a Splicing Mutation
12 Analysis of Common Splicing Problems
13 Ultracentrifugation in the Analysis and Purification of Spliceosomes Assembled In Vitro
14 Chemical Synthesis of RNA
15 RNA Interference (siRNA, shRNA)
16 Expression and Purification of Splicing Proteins
17 Detection of RNA–Protein Complexes by Electrophoretic Mobility Shift Assay
18 Functional Analysis of Large Exonic Sequences Through Iterative In Vivo Selection
19 Identification of Splicing cis-Elements Through an Ultra-Refined Antisense Microwalk
20 Genomic SELEX to Identify RNA Targets of Plant RNA-Binding Proteins
21 Quantification of Alternative Splice Variants
22 High-Throughput Analysis of Alternative Splicing by RT-PCR
23 Monitoring Changes in Plant Alternative Splicing Events
24 Array Analysis
25 The CLIP Method to Study Protein–RNA Interactions in Intact Cells and Tissues
26 RNA–Protein Crosslinking and Immunoprecipitation (CLIP) in Schizosaccharomyces pombe
27 Identification of Proteins Bound to RNA
28 Single-Cell Detection of Splicing Events with Fluorescent Splicing Reporters
29 The Preparation of HeLa Cell Nuclear Extracts
30 In Vitro Splicing Assays
31 Assembly and Isolation of Spliceosomal Complexes In Vitro
32 Analysis of Site-Specific RNA–Protein Interactions
33 Immunoprecipitation and Pull-Down of Nuclear Proteins
34 Analysis of Protein (-RNA) Complexes by (Quantitative) Mass Spectrometric Analysis
35 Fast Cloning of Splicing Reporter Minigenes
36 In Vivo Analysis of Splicing Assays
37 Coupled Promoter Splicing Systems
38 Stable Cell Lines with Splicing Reporters
39 Splicing Factor ChIP and ChRIP: Detection ofSplicing and Splicing Factors at
Genes by Chromatin Immunoprecipitation
40 Yeast Genetics to Investigate the Function of Core Pre-mRNA Splicing Factors
41 Analysis of HIV-1 RNA Splicing
42 In Vivo Analysis of Plant Intron Splicing
43 Modification State-Specific Antibodies
44 Analysis of Alternative Splicing in Drosophila Genetic Mosaics
45 Antisense Derivatives of U7 Small Nuclear RNA as Modulators of Pre-mRNA Splicing
46 Screening for Alternative Splicing Modulators
47 Use of Oligonucleotides to Change Splicing
48 Changing Signals to the Spliceosome
49 Overview of Splicing Relevant Databases
50 Analysis of RNA Transcripts by High-Throughput RNA Sequencing
51 Identification of Splicing Factor Target Genes by High-Throughput Sequencing
52 Bioinformatic Analysis of Splicing Events
53 Analysis of Pre-mRNA Secondary Structures and Alternative Splicing
54 Structure Prediction for Alternatively Spliced Proteins
55 Comparative Genomics Methods for the Prediction of Small RNA-Binding Sites