Arthritis Research Volume 2 Methods and Protocols 1st Edition by Andrew P Cope ISBN 1597454028 9781597454025 by Andrew P. Cope 158829918X instant download after payment.

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ISBN 10: 1597454028

ISBN 13: 9781597454025 

Author: Andrew P. Cope

Arthritis Research: Methods and Protocols is a compendium of data pertinent to the methods and protocols that have contributed to recent advances in molecular medicine in general, but to the molecular basis of rheumatic disease in particular. These volumes details novel technologies, some of which are still evolving and whose impacts are yet to be determined. Leaders in the field contribute to cover such exciting and cutting edge topics as imaging and immunohistochemistry, analysis of cartilage and bone catabolism, immunobiology, and cell trafficking.

In Volume 1, authors discuss synovial joint morphology, histopathology, and immunohistochemistry, cartilage matrix and bone biology, and cell trafficking, migration and invasion. Volume 2 is broken up into sections that cover immunobiology and T cells, animal models of arthritis, and applications of new technologies, such as Differential Display Reverse Transcription-Polymerase Chain Reaction (DDRT-PCR), to define novel therapeutic targets. Both volumes combine to produce a concise set of protocols condensing decades of experience and expertise.

Arthritis Research: Methods and Protocols will be a valuable tool for basic research investigators, clinician scientists, pathologists, immunologists, and biochemists looking to stay current in their fields.

Table of contents:

1 Front Matter

Immunobiology
2. Phenotypic Analysis of B-Cells and Plasma Cells
3. Detection of Antigen Specific B-Cells in Tissues
4. Single Cell Analysis of Synovial Tissue B-Cells
5. Tracking Antigen Specific CD4+ T-Cells With Soluble MHC Molecules
6. Analysis of Antigen Reactive T-Cells
7. Identification and Manipulation of Antigen Specific T-Cells with Artificial Antigen Presenting Cells
8. Analysis of Th1/Th2 T-Cell Subsets
9. Analysis of the T-Cell Receptor Repertoire of Synovial T-Cells
10. The Assessment of T-Cell Apoptosis in Synovial Fluid
11. Assays of T-Cell Contact Dependent Monocyte-Macrophage Functions
12. Phenotypic and Functional Analysis of Synovial Natural Killer Cells
13. Identification and Isolation of Synovial Dendritic Cells

Animal Models of Arthritis
14. The Use of Animal Models for Rheumatoid Arthritis
15. Collagen-Induced Arthritis in Mice
16. Collagen-Induced Arthritis in Rats
17. Collagen Antibody Induced Arthritis
18. Arthritis Induced with Minor Cartilage Proteins
19. Murine Antigen-Induced Arthritis
20. Pristane-Induced Arthritis in the Rat
21. The K/BxN Mouse Model of Inflammatory Arthritis
22. Analysis of Arthritic Lesions in the Del1 Mouse

Application of New Technologies to Define Novel Therapeutic Targets
23. Gene Expression Profiling in Rheumatology
24. Differential Display Reverse Transcription-Polymerase Chain Reaction to Identify Novel Biomolecules in Arthritis Research
25. Two-Dimensional Electrophoresis of Proteins Secreted from Articular Cartilage
26. Mapping Lymphocyte Plasma Membrane Proteins
27. In Vivo Phage Display Selection in the Human/SCID Mouse Chimera Model for Defining Synovial Specific Determinants
28. Adenoviral Targeting of Signal Transduction Pathways in Synovial Cell Cultures

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