Calcium Signaling Protocols Methods in Molecular Biology 2nd Edition by David Lambert 161737573X 978-1617375736 by David G. Lambert 1588294420 instant download after payment.

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Product details:

ISBN 10: 161737573X

ISBN 13: 978-1617375736

Author: David G. Lambert

In the first edition of Calcium Signaling Protocols I began by writing “The regula- 2+ tion of intracellular Ca is a common theme presented in many papers over the last 20 2+ or so years and the description of the Ca -sensitive indicator dye fura-2 in 1985 resulted in a massive increase in these types of studies. ” This statement is as true in 2005 as it was in 1999, but 20 or so years is now 30 years! There has been some reorganization of the volume such that there are now 22 ch- ters including five new ones, all written by experts in their field. These new chapters 2+ include use of the FlexStation and electrophysiological measurement of Ca channel activity. The book is broken into six parts. Part I is a general coverage of basic theory and the simplest use of fluorescent indicators. Part II covers specialist measurement 2+ systems and Part III covers measurement of Ca channel activity. Assessment of 2+ release of stored Ca is covered in some detail in Part IV, with Parts V and VI cover- 2+ ing specialist measurement techniques and Ca -sensitive targets. Putting a book like this together, even as a second edition, takes time and I am, again, indebted to the individual authors for their help and patience. I am also very grateful to Professor John M. Walker, the series editor, for his continued help and advice over the course of this project.

Table of contents:

1. Fluorescent Measurement of [Ca²⁺]c

2. Measurement of [Ca²⁺]i in Whole Cell Suspensions Using Fura-2

3. Measurement of [Ca²⁺]i in Cell Suspensions Using Indo-1

4. Confocal Microscopy

5. Single-Cell and Subcellular Measurement of Intracellular Ca²⁺ Concentration

6. Ratiometric Ca²⁺ Measurements Using the FlexStation® Scanning Fluorometer

7. Measuring Ca²⁺ Changes in Multiwell Format Using the Fluorometric Imaging Plate Reader

8. Measurement of Ca²⁺ Entry Using ⁴⁵Ca²⁺

9. Measurement of [³H]PN200-110 and [¹²⁵I]ω-Conotoxin MVIIA Binding

10. Whole-Cell Patch Clamp Recording of Voltage-Sensitive Ca²⁺ Channel Currents

11. Measurement of Phospholipase C by Monitoring Inositol Phosphates Using [³H]Inositol-Labeling Protocols in Permeabilized Cells

12. Measurement of Inositol(1,4,5)Trisphosphate Using a Stereospecific Radioreceptor Mass Assay

13. Measurement of Calcium Fluxes in Permeabilized Cells Using a ⁴⁵Ca²⁺ Uptake and Release Assay

14. Microinjection of myo-Inositol(1,4,5)Trisphosphate and Other Calcium-Mobilizing Agents Into Intact Adherent Cells

15. Measurement of Free [Ca²⁺] Changes in Agonist-Sensitive Internal Stores Using Compartmentalized Fluorescent Indicators

16. Measurement of [Ca²⁺]i in Smooth Muscle Strips Using Front-Surface Fluorimetry

17. Measurement of Calcium and Movement in Heart Cells

18. Simultaneous Analysis of Intracellular pH and Ca²⁺ From Cell Populations

19. Measurement of Cytosolic-Free Ca²⁺ in Plant Tissue

20. Assay and Purification of Calmodulin-Dependent Protein Kinase

21. Assay and Purification of Calmodulin-Dependent Cyclic Nucleotide Phosphodiesterase and Isozyme Separation

22. Measurement of Ca²⁺-ATPase Activity (in PMCA and SERCA1)

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Tags: David Lambert, Calcium, Signaling, Protocols