Cancer Cell Signaling Methods And Protocols 1st Edition Erik S Knudsen by Erik S. Knudsen, Steven P. Angus (auth.), David M. Terrian (eds.) 9781588290755, 9781592593569, 1588290751, 1592593569 instant download after payment.

The rapidly evolving nature of cell signaling research in cancer has necessitated a continuous updating of the methods used in its study, most recently to take into account the analysis of multiple members within a gene family. Cancer Cell Signaling: Methods and Protocols brings together all the major methods of cell research that are scientifically grounded within the cancer biology field. Presented in step-by-step detail to ensure successful results, the methods include proven techniques for the investigation of apoptosis and cell death, complementary protocols for manipulating and/or monitoring oncogenic signals in cancer cells, and techniques for studying protein-protein interactions. Finally, there is a detailed protocol for capturing pure samples of malignant cells from frozen tissue specimens and two alternative techniques for analyzing their genomic DNA. Each method is described by a hands-on master of the technique and includes invaluable notes on troubleshooting and pitfalls to avoid.
Cutting-edge and highly practical, Cancer Cell Signaling: Methods and Protocols provides today's cancer researchers with new, but well-proven, strategies and procedures for investigating how the signaling cascades essential to normal cell life are corrupted, as well as for developing new molecular tools for cancer therapy.