Celiac Disease Methods And Protocols 1st Edition Michael N Marsh by Michael N. Marsh 9780896036505, 0896036502 instant download after payment.

With the recent development of powerful bioanalytical techniques, research on gluten sensitivity has entered an exciting new phase. In Celiac Disease: Methods and Protocols, Professor Michael N. Marsh, a recognized world authority on this condition, together with a team of other expert laboratory/clinical investigators from around the world, present a collection of these state-of-the-art techniques for studying the biology and immunopathology of celiac disease. Both novice and experienced researchers will find in this collection detailed step-by-step methods for cloning lymphocytes, creating gene/peptide libraries, and performing genotyping, linkage, and positional cloning. Also included are techniques for determining the peptide structure of HLA-bound material (tandem mass spectroscopy), computerized morphometry, in situ hybridization, organ culture, and monoclonal AB assays.Celiac Disease: Methods and Protocols provides all experimental and clinical investigators with the essential core of readily reproducible methods necessary for successful work on celiac disease today.