Drug Nucleic Acid Interactions 1st Edition by Jonathan B Chaires, Michael J Waring ISBN 0121822419 9780121822415 by Jonathan B. Chaires, Michael J. Waring 9780080496900, 9780121822415 instant download after payment.

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ISBN 10: 0121822419 
ISBN 13: 9780121822415
Author: Jonathan B Chaires, Michael J Waring

Drug Nucleic Acid Interactions 1st Table of contents:

• Part I: Structural and Biophysical Approaches

  • Chapter 1: Spectroscopic Methods for Characterizing Drug-DNA Interactions
    • UV-Vis Spectroscopy (Absorption changes, hypochromism, hyperchromism)
    • Fluorescence Spectroscopy (Fluorescence quenching, energy transfer, competitive binding assays)
    • Circular Dichroism (CD) Spectroscopy (Conformational changes in DNA/RNA upon drug binding)
    • Infrared and Raman Spectroscopy (Vibrational modes, specific bond interactions)
  • Chapter 2: Isothermal Titration Calorimetry (ITC) for Studying Drug-Nucleic Acid Binding
    • Principles of ITC
    • Experimental setup and data analysis
    • Determining binding stoichiometry, affinity, and thermodynamic parameters (ΔH,ΔS,ΔG)
  • Chapter 3: Surface Plasmon Resonance (SPR) for Analyzing Drug-Nucleic Acid Kinetics
    • Principles of SPR
    • Immobilization of nucleic acids
    • Kinetic analysis (association and dissociation rates, ka​,kd​,KD​)
    • Concentration determination
  • Chapter 4: Molecular Dynamics Simulations of Drug-DNA/RNA Complexes
    • Introduction to MD simulations in drug discovery
    • Setting up simulations (force fields, solvation)
    • Analysis of trajectories (RMSD, hydrogen bonds, interaction energies)
    • Predicting binding modes and stability
  • Chapter 5: Nuclear Magnetic Resonance (NMR) Spectroscopy in Drug-Nucleic Acid Studies
    • 2D NMR techniques (NOESY, COSY, HSQC)
    • Structural determination of drug-nucleic acid complexes
    • Ligand-based NMR (e.g., STD-NMR, Chemical Shift Perturbation) for fragment screening and binding site mapping
  • Chapter 6: Electrophoretic Mobility Shift Assay (EMSA) for DNA/RNA Binding
    • Principles of EMSA (gel shift assay)
    • Detection of binding, stoichiometry
    • Competition assays
  • Chapter 7: Atomic Force Microscopy (AFM) for Visualizing Drug-Nucleic Acid Interactions
    • Imaging drug-induced conformational changes in nucleic acids
    • Quantifying binding events at the single-molecule level

• Part II: Targeted Interactions and Functional Assays

  • Chapter 8: Targeting G-Quadruplex DNA with Small Molecules
    • Introduction to G-quadruplex structures and their biological relevance
    • Methods for G-quadruplex formation and characterization
    • Ligand binding assays specific to G-quadruplexes
  • Chapter 9: Investigation of DNA Intercalation by Small Molecules
    • Classic intercalation assays (viscosity, melting temperature, unwinding assays)
    • Fluorescence displacement assays
  • Chapter 10: Minor Groove Binding Drug Interactions with DNA
    • Characterization methods for minor groove binders (e.g., footprinting, displacement assays)
    • Sequence specificity of minor groove binding
  • Chapter 11: DNA Alkylating Agents: Methods for Studying DNA Damage
    • Techniques for detecting DNA adducts
    • Assays for DNA cross-linking and strand breaks
  • Chapter 12: Ribozyme-Drug Interactions
    • Methods for studying drug binding to catalytic RNA (ribozymes)
    • Functional assays for inhibition or activation of ribozyme activity
  • Chapter 13: Aptamers as Therapeutic Agents and Tools for Drug Discovery
    • SELEX (Systematic Evolution of Ligands by EXponential enrichment) for aptamer discovery
    • Characterization of aptamer-drug interactions
    • Aptamer-based sensors

• Part III: Cellular and Clinical Implications

  • Chapter 14: Cellular Uptake and Localization of Nucleic Acid-Targeting Drugs
    • Fluorescence microscopy and flow cytometry for cellular studies
    • Confocal imaging of drug distribution
  • Chapter 15: Assessing Drug-Induced DNA Damage in Cells (Comet Assay, γH2AX Staining)
    • Techniques for quantifying DNA damage in living cells
    • Monitoring DNA repair pathways
  • Chapter 16: Resistance Mechanisms to Nucleic Acid-Targeting Drugs
    • Molecular mechanisms of drug resistance (e.g., efflux pumps, target modification)
    • Methods for identifying and characterizing resistance
  • Chapter 17: Translational Aspects: From Bench to Bedside
    • Challenges in developing nucleic acid-targeting drugs
    • Biomarkers for efficacy and toxicity
    • Future directions in drug-nucleic acid interaction research

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Tags: Jonathan B Chaires, Michael J Waring, Drug, Nucleic