Flow Cytometry Cells From Without Leukocytes Surface Proteins And The Strategy Of Gating 2nd Edition Alice Longobardi Givan by Alice Longobardi Givan 9780471382249, 0471382248 instant download after payment.

Chapter Six (“Cells from Without: Leukocytes, Surface Proteins, and the Strategy of Gating”) introduces phenotyping of leukocytes, a major application of flow cytometry. Cells from blood are described along with methods for separating leukocytes from erythrocytes and for using antibodies to stain leukocytes for surface proteins. Explanations are given of the use of controls, of the quantitation of fluorescence with beads, and of the factors that affect the sensitivity of these measurements. Finally, gating of leukocytes for analysis is described, including gating on scatter signals, back-gating to adjust the position of a gate, assessing the purity and inclusivity of a gate, and newer strategies using multi-parameter fluorescence to avoid some of the ambiguities of traditional gating.
10.1002/0471223948.ch6