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EbookBell Team
4.1
30 reviewsWith contributions from leading international authorities, this book equips readers with clear and detailed protocols for both well-known and up-and-coming techniques along with hints and tips for success. All the methods have been tried and tested by leading international research labs and are presented in easy-to-follow stages along with a useful overview of each technique.
This book is an essential resource for all researchers in molecular biology, biochemistry, pharmacology and for graduate students.Content:
Chapter 1 Measurement of Ligand–G Protein?Coupled Receptor Interactions (pages 1–29): Katie Leach, Celine Valant, Patrick M. Sexton and Arthur Christopoulos
Chapter 2 Second Messenger Assays for G Protein?Coupled Receptors: cAMP, Ca2+, Inositol Phosphates, ERK1/2 (pages 31–52): Karen J. Gregory, Patrick M. Sexton, Arthur Christopoulos and Caroline A. Hick
Chapter 3 Use of the [35S]GTP?S Binding Assay to Determine Ligand Efficacy at G Protein?Coupled Receptors (pages 53–68): Elodie Kara and Philip G. Strange
Chapter 4 Quantitative Imaging of Receptor Trafficking (pages 69–83): Andy R. James, Takeo Awaji, F. Anne Stephenson and Nicholas A. Hartell
Chapter 5 Production of Recombinant G Protein?Coupled Receptor in Yeast for Structural and Functional Analysis (pages 85–110): Richard A. J. Darby, Mohammed Jamshad, Ljuban Grgic, William J. Holmes and Roslyn M. Bill
Chapter 6 Monitoring GPCR–Protein Complexes Using Bioluminescence Resonance Energy Transfer (pages 111–132): Werner C. Jaeger, Kevin D. G. Pfleger and Karin A. Eidne
Chapter 7 Using Intramolecular Fluorescence Resonance Energy Transfer to Study Receptor Conformation (pages 133–146): Cornelius Krasel and Carsten Hoffmann
Chapter 8 A Disulfide Cross?linking Strategy Useful for Studying Ligand?induced Structural Changes in GPCRs (pages 147–167): Jian Hua Li, Stuart D. C. Ward, Sung?Jun Han, Fadi F. Hamdan and Jurgen Wess
Chapter 9 Use of Fluorescence Correlation Spectroscopy to Study the Diffusion of G Protein?coupled Receptors (pages 169–195): Stephen J. Briddon, Jonathan A. Hern and Stephen J. Hill
Chapter 10 Identification and Analysis of GPCR Phosphorylation (pages 197–214): Kok Choi Kong, Sharad C. Mistry and Andrew B. Tobin
Chapter 11 Measurement and Visualization of G Protein?coupled Receptor Trafficking by Enzyme?linked Immunosorbent Assay and Immunofluorescence (pages 215–228): Stuart J. Mundell, Shaista P. Nisar and Eamonn Kelly
Chapter 12 Substituted Cysteine Accessibility Method (SCAM) (pages 229–250): George Liapakis and Jonathan A. Javitch
Chapter 13 Homology Modelling of G Protein?Coupled Receptors (pages 251–273): John Simms
