High Throughput Mass Spectrometry In Drug Discovery Chang Liu by Chang Liu, Hui Zhang 9781119678434, 1119678439 instant download after payment.

This book starts with an overview of the 40 years of efforts to improve the analytical throughput of MS-based
approaches (Chapter 1). Then, technologies with high-speed sequential and parallel chromatographic sample introduction, high repetition rate lasers, ion mobility, and low-volume MS samplings were summarized. Due to its high specificity and high sensitivity, the LC-MS technology has been widely used in various steps of the drug discovery workflow. In Part 2 (Chapter 2–3), the efforts to improve the LC-MS analytical throughput are introduced. The development of the high-speed sample introduction for LC-MS and its application on ADME and HTS applications is described in Chapter 2. Another approach for throughput improvement utilizing paralleled multiplexing LC is described in Chapter 3. Following the conventional LC-MS-based technologies, other electrospray ionization (ESI)MS-based high-throughput platforms without chromatographic separation are summarized in Part 3 (Chapter 4–7). Direct online solid-phase extraction (SPE) MS and its application in ADME and HTS workflows are described in Chapter 4. The utilization of the acoustic energy for non-contact transfer samples from microplates to MS for high-throughput analysis, including the acoustic mist ionization (AMI) and through the open-port interface (OPI), is
summarized in Chapter 5. By skipping the chromatographic separation process, these approaches demonstrated higher analytical throughput than the conventional LC-MS approach. However, there would be the risk of potential isomeric/isobaric interference. Ion mobility spectrometry (IMS) and differential mobility spectrometry (DMS), described in Chapters 6 and 7, respectively, provide the additional dimension of the selectively, potentially solving the specificity issues of these high-throughput technologies for some drug discovery assays.