Most ebook files are in PDF format, so you can easily read them using various software such as Foxit Reader or directly on the Google Chrome browser.
Some ebook files are released by publishers in other formats such as .awz, .mobi, .epub, .fb2, etc. You may need to install specific software to read these formats on mobile/PC, such as Calibre.
Please read the tutorial at this link: https://ebookbell.com/faq
We offer FREE conversion to the popular formats you request; however, this may take some time. Therefore, right after payment, please email us, and we will try to provide the service as quickly as possible.
For some exceptional file formats or broken links (if any), please refrain from opening any disputes. Instead, email us first, and we will try to assist within a maximum of 6 hours.
EbookBell Team
5.0
20 reviewsCITATION
Wang, S., Yu, M., Wang, Z., Li, J., Liu, C., Liu, D., ... & Wang, R. (2025). Integrated Multiscale Imaging and Noninvasive Micro‐Sensing Decipher Spatiotemporal Calcium Dynamics in Thermogenic Tissue of Magnolia Flower. Plant, Cell & Environment. First published: 23 September 2025
ABSTRACTMagnolia denudata is characterized by floral thermogenesis and blooms in the cold early spring. However, the specific thermogenic tissues and molecular signals that modulate thermogenesis remain elusive. Here, we categorized the developmentalprocess of M. denudata into five stages with stage 2 being the thermogenic peak stage, and identified the thermogenic region asthe lateral tissue of gynoecium by integrating infrared (IR) imaging and multispectral imaging (MSI). The optimized integrationof these imaging techniques not only distinguished the gynoecium of the non‐thermogenic and thermogenic stages but alsorevealed compound differences between the lateral and central tissues at the thermogenic stage. Moreover, we unveiled thein situ distribution of calcium in thermogenic organs using micro X‐ray fluorescence imaging (μ‐XRF), and its distributionpattern closely matched the heat distribution. The increased rate of Ca2+ influx both into the cytosol and mitochondria alignswith the upregulation of genes related to mitochondrial Ca2+ transport at the thermogenic stage. Additionally, changes inrespiratory capacity caused by altering cytosolic Ca2+ concentration further demonstrated that Ca2+ regulates mitochondrialrespiratory metabolism. This study comprehensively utilized multiscale imaging to distinguish the thermogenic tissue withinthe complex‐structured thermogenic organ of M. denudata, revealing the close relationship between Ca2+ and thermogenesis.
