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ISBN 10: 1119156785
ISBN 13: 978-1119156789
Author: Seung-Beom Hong, M. Bazlur Rashid, Lory Z. Santiago-Vázquez
Part I: Methods in Biotechnology (MB) Laboratory Exercises
1 - MB experiment 1: Lab measurements
2 - MB experiment 2: Use of the spectrophotometer and Beer’s law
3 - MB experiment 3: Making solutions and buffer efficacy
4 - MB experiment 4: Acid–base titration
5 - MB experiment 5: Protein denaturation and precipitation
6 - MB experiment 6: Bacterial transformation
7 - MB experiment 7: GFP purification
8 - MB experiment 8: SDS-PAGE analysis
9 - MB experiment 9: DNA isolation
10 - MB experiment 10: PCR-based Alu-human DNA typing
11 - MB experiment 11: Restriction enzyme digestion
12 - MB experiment 12: Agarose gel electrophoresis
13 - MB experiment 13: Ouchterlony and ELISA immunoassays
14 - MB experiment 14: Testing plant substances for antimicrobial activity
15 - MB experiment 15: Peroxidase enzyme activity assay
Part II: Advanced Methods in Biotechnology (AMB) 1 Laboratory Exercises
16 - AMB 1 experiment 16: Aseptic technique and culture handling
17 - AMB 1 experiment 17: Yeast culture media preparation
18 - AMB 1 experiment 18: Growth curve
19 - AMB 1 experiment 19: Mini plasmid prep
20 - AMB 1 experiment 20: Restriction digestion, purification concentration and quantification of DNA
21 - AMB 1 experiment 21: Polymerase chain reaction (PCR)
22 - AMB 1 experiment 22: TA blunt end SLIC, and CPEC cloning of PCR product
23 - AMB 1 experiment 23: One-step multifragment assembly cloning
24 - AMB 1 experiment 24: Restriction enzyme digestion and fast agarose gel electrophoresis
25 - AMB 1 experiment 25: Southern blot transfer
26 - AMB 1 experiment 26: Probe labeling and purification
27 - AMB 1 experiment 27: Dot blot assay
28 - AMB 1 experiment 28: Pre-hybridization, hybridization and detection
29 - AMB 1 experiment 29: Total yeast RNA isolation and RT-PCR
30 - AMB 1 experiment 30: Yeast-based in vivo recombination cloning
31 - AMB 1 experiment 31: Plasmid DNA isolation from yeast
32 - AMB 1 experiment 32: E. coli transformation with yeast plasmid DNA
33 - AMB 1 experiment 33: X-gal filter lift assay
34 - AMB 1 experiment 34: Protein quantitation assay
35 - AMB 1 experiment 35: Quantitative 𝛃-Galactosidase assay in yeast
36 - AMB 1 experiment 36: Gel filtration chromatography (GFC)
37 - AMB 1 experiment 37: Ion exchange chromatography (IEC)
Part III: Advanced Methods in Biotechnology (AMB) 2 Laboratory Exercises
38 - AMB 2 experiment 38: E. coli culture media preparation
39 - AMB 2 experiment 39: Site-directed mutagenesis
40 - AMB 2 experiment 40: Protein expression in E. coli
41 - AMB 2 experiment 41: Protein purification by affinity column chromatography
42 - AMB 2 experiment 42: SDS-PAGE analysis of affinity column fractions
43 - AMB 2 experiment 43: Western blot analysis of affinity column fractions
44 - AMB 2 experiment 44: Yeast media preparation and phenotypic analysis of yeast strains
45 - AMB 2 experiment 45: Yeast transformation for yeast two-hybrid (Y2H) assay and genome editing by CRISPR-Cas system
46 - AMB 2 experiment 46: Yeast mating-mediated Y2H assay and genomic PCR
47 - AMB 2 experiment 47: Yeast colony PCR screening and cycle DNA sequencing
48 - AMB 2 experiment 48: DNA sequencing electrophoresis
49 - AMB 2 experiment 49: RNA interference
50 - AMB 2 experiment 50: Protein preparation for 2D gel electrophoresis
51 - AMB 2 experiment 51: Two-dimensional gel electrophoresis
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Tags: Seung Beom Hong, M Bazlur Rashid, Lory Z Santiago Vazquez, Methods, Biotechnology
