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Methods to Study Litter Decomposition A Practical Guide 1st Edition by Manuel A S Graca, Felix Barlocher, Mark O Gessner ISBN 1402033486 9781402033483

  • SKU: BELL-2136086
Methods to Study Litter Decomposition A Practical Guide 1st Edition by Manuel A S Graca, Felix Barlocher, Mark O Gessner ISBN 1402033486 9781402033483
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Methods to Study Litter Decomposition A Practical Guide 1st Edition by Manuel A S Graca, Felix Barlocher, Mark O Gessner ISBN 1402033486 9781402033483 instant download after payment.

Publisher: Springer
File Extension: PDF
File size: 6.21 MB
Pages: 314
Author: Manuel A.S. Graça, Felix Bärlocher, Mark O. Gessner
ISBN: 1402033486, 9781402033483
Language: English
Year: 2005
Edition: 1

Product desciption

Methods to Study Litter Decomposition A Practical Guide 1st Edition by Manuel A S Graca, Felix Barlocher, Mark O Gessner ISBN 1402033486 9781402033483 by Manuel A.s. Graça, Felix Bärlocher, Mark O. Gessner 1402033486, 9781402033483 instant download after payment.

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Product details:

ISBN 10: 1402033486 
ISBN 13: 9781402033483
Author: Manuel A S Graca, Felix Barlocher, Mark O Gessner

Decomposition of organic matter is a major ecosystem process involving an array of different organisms, including bacteria, fungi and invertebrates. The main objective of this book is to provide students and laboratory instructors at universities and professional ecologists with a broad range of established methods to study plant litter decomposition. Detailed protocols for direct use in the field or laboratory are presented in an easy to follow step-by-step format. A short introduction to each protocol reviews the ecological significance and principles of the technique and points to key references. Although most methods are described for freshwater ecosystems, many will work equally well in the presented or slightly modified form for studies in marine and terrestrial environments.

Methods to Study Litter Decomposition A Practical Guide 1st Table of contents:

Part I: Introduction and General Concepts

  • Chapter 1: The Significance of Litter Decomposition in Ecosystems
  • Chapter 2: Principles of Litter Decomposition
  • Chapter 3: Experimental Design in Litter Decomposition Studies
  • Chapter 4: Statistical Analysis for Litter Decomposition Data (Often includes a primer on relevant statistical methods)

Part II: Preparation and Characterization of Litter

  • Chapter 5: Litter Collection and Preparation
  • Chapter 6: Determination of Litter Mass Loss (Litter Bag Technique)
  • Chapter 7: Coarse Particulate Organic Matter (CPOM) Budgets
  • Chapter 8: Fine Particulate Organic Matter (FPOM) Collection
  • Chapter 9: Leaching of Soluble Compounds
  • Chapter 10: Determination of Leaf Toughness
  • Chapter 11: Assessing Physical Properties of Litter
  • Chapter 12: Chemical Analysis of Litter: Overview
  • Chapter 13: Determination of Total Nitrogen and Phosphorus in Leaf Litter
  • Chapter 14: Analysis of Carbon Content
  • Chapter 15: Determination of Total Carbohydrates
  • Chapter 16: Determination of Soluble Carbohydrates
  • Chapter 17: Total Lipids
  • Chapter 18: Total Phenolics
  • Chapter 19: Radial Diffusion Assay for Tannins
  • Chapter 20: Acid Butanol Assay for Proanthocyanidins (Condensed Tannins)
  • Chapter 21: Proximate Lignin and Cellulose Determination
  • Chapter 22: Other Specific Chemical Analyses (e.g., cell wall components, specific nutrients)

Part III: Microbial Decomposers: Fungi

  • Chapter 23: Techniques for Handling Aquatic Hyphomycetes
  • Chapter 24: Maintenance of Aquatic Hyphomycete Cultures
  • Chapter 25: An Illustrated Key to Common Temperate Species of Aquatic Hyphomycetes
  • Chapter 26: Sporulation by Aquatic Hyphomycetes (Quantification and Rate)
  • Chapter 27: Ergosterol as a Measure of Fungal Biomass
  • Chapter 28: Acetate Incorporation into Ergosterol to Determine Fungal Growth Rates and Production
  • Chapter 29: Molecular Approaches to Estimate Fungal Diversity I: T-RFLP
  • Chapter 30: Molecular Approaches to Estimate Fungal Diversity II: DGGE
  • Chapter 31: Next-Generation Sequencing (NGS) for Fungal Communities
  • Chapter 32: Fungal Biomarkers Beyond Ergosterol
  • Chapter 33: Isolation and Characterization of Litter-Associated Fungi

Part IV: Microbial Decomposers: Bacteria

  • Chapter 34: Bacterial Counts and Biomass Determination by Epifluorescence Microscopy
  • Chapter 35: Secondary Production and Growth of Litter-Associated Bacteria
  • Chapter 36: Isolation of Cellulose-Degrading Bacteria
  • Chapter 37: Molecular Approaches for Bacterial Community Analysis (e.g., NGS)
  • Chapter 38: Extraction and Quantification of ATP as a Measure of Microbial Biomass

Part V: Microbial Activity and Extracellular Enzymes

  • Chapter 39: Respirometry for Measuring Microbial Activity
  • Chapter 40: Extracellular Fungal Hydrolytic Enzyme Activity: General Principles
  • Chapter 41: Assays for Cellulases (e.g., Viscosimetric, Spectrophotometric)
  • Chapter 42: Fluorometric Determination of the Activity of β-Glucosidase and Other Extracellular Hydrolytic Enzymes
  • Chapter 43: Pectin-Degrading Enzymes: Polygalacturonase and Pectin Lyase
  • Chapter 44: Lignin-Degrading Enzymes: Phenoloxidase and Peroxidase
  • Chapter 45: Proteinase Activity: Azocoll and Thin-Layer Enzyme Assay
  • Chapter 46: Other Relevant Enzyme Assays

Part VI: Detritivorous Consumers (Invertebrates)

  • Chapter 47: Maintenance of Shredders in the Laboratory
  • Chapter 48: Measuring Feeding Preferences of Detritivores
  • Chapter 49: Assessing Invertebrate Ingestion and Assimilation Rates
  • Chapter 50: Characterization of Invertebrate Assemblages in Litter
  • Chapter 51: Tracing Trophic Pathways (e.g., Stable Isotopes)
  • Chapter 52: Influence of Invertebrates on Litter Fragmentation
  • Chapter 53: Methods for Exclosure Experiments

Part VII: Ecosystem-Level and Integrative Approaches

  • Chapter 54: Litter Dynamics at the Stream Ecosystem Level
  • Chapter 55: Whole-Stream/Ecosystem Manipulations
  • Chapter 56: Using Litter Decomposition as a Bioindicator
  • Chapter 57: Modelling Litter Decomposition
  • Chapter 58: Data Synthesis and Meta-Analysis in Decomposition Studies
  • Chapter 59: Biodiversity and Litter Decomposition (Specific Methods)
  • Chapter 60: Applications of Litter Decomposition Research (e.g., Climate Change, Pollution)

Appendices

  • Glossary of Terms
  • List of Suppliers
  • Online Resources and Data (e.g., computer code, calculation sheets mentioned in descriptions)
  • Detailed Protocols (often integrated into chapters)

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Tags: Manuel A S Graca, Felix Barlocher, Mark O Gessner, Methods, Litter

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