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Molecular Motors Methods and Protocols Methods in Molecular Biology Vol 392 1st Edition by Ann Sperry 1588296652 978-1588296658

  • SKU: BELL-2175112
Molecular Motors Methods and Protocols Methods in Molecular Biology Vol 392 1st Edition by Ann Sperry 1588296652 978-1588296658
$ 31.00 $ 45.00 (-31%)

4.8

64 reviews

Molecular Motors Methods and Protocols Methods in Molecular Biology Vol 392 1st Edition by Ann Sperry 1588296652 978-1588296658 instant download after payment.

Publisher: Humana Press
File Extension: PDF
File size: 23.51 MB
Pages: 240
Author: Ann O. Sperry
ISBN: 9781588296658
Language: English
Year: 2007
Edition: 1

Product desciption

Molecular Motors Methods and Protocols Methods in Molecular Biology Vol 392 1st Edition by Ann Sperry 1588296652 978-1588296658 by Ann O. Sperry 9781588296658 instant download after payment.

Molecular Motors Methods and Protocols Methods in Molecular Biology Vol 392 1st Edition by Ann Sperry - Ebook PDF Instant Download/Delivery: 1588296652, 978-1588296658

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Product details:

ISBN 10: 1588296652

ISBN 13: 978-1588296658

Author: Ann O. Sperry

Molecular motor proteins comprise three protein superfamilies: kinesins, dyneins and myosins. Together, these proteins produce force for movement in an incredibly wide variety of cellular processes including vesicular transport, cell division, nuclear migration, muscle contraction, mechanochemical transduction, among others. In addition, molecular motors are expressed in both simple eukaryotes and in a tissue specific manner in higher cells. This methods volume explores the extreme functional and structural diversity of molecular motors and presents methods relevant to each motor family. In addition, it describes techniques directed at motors that fall outside of the 3 characterized families (i.e. dynamin and F1ATPase). This volume of the Methods in Molecular Biology series is an essential reference for cell biologists examining the function of motor proteins in a range of model organisms using both in vitro and in vivo techniques. This volume is a valuble source for biochemists and molecular biologists as well.

Table of contents:

  1. Equilibrium Binding of Proteins to F-Actin

  2. Analysis of Calcium/Calmodulin Regulation of a Plant Kinesin Using Co-Sedimentation and ATPase Assays

  3. In Vitro and In Vivo Analysis of Microtubule-Destabilizing Kinesins

  4. Approaches to Kinesin-1 Phosphorylation

  5. Protein Modification to Probe Intradynein Interactions and In Vivo Redox State

  6. Methods to Study the Interactions of the Dynein Light Chains and Intermediate Chains

  7. Identification of Motor Protein Cargo by Yeast 2-Hybrid and Affinity Approaches

  8. In Situ Binding Assay to Detect Myosin-1c Interactions with Hair-Cell Proteins

  9. Ultrastructural Analysis of Kinesin-Related Motor Proteins During Spermatogenesis

  10. In Vitro Motility System to Study the Role of Motor Proteins in Receptor-Ligand Sorting

  11. Enrichment and Disassembly of Ectoplasmic Specializations in the Rat Testis

  12. Single-Molecule Observation of Rotation of F1-ATPase Through Microbeads

  13. The Use of FRET in the Analysis of Motor Protein Structure

  14. Structure Determination of the Motor Domain of Yeast Kinesin Kar3 by X-Ray Crystallography

  15. High-Resolution Structural Analysis of the Kinesin-Microtubule Complex by Electron Cryo-Microscopy

  16. Chemical-Genetic Inhibition of Sensitized Mutant Unconventional Myosins

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