Multiomicsbased Mapping Of Decidualization Resistance In Patients With A History Of Severe Preeclampsia Teresa Cordero1 by Teresa Cordero1, Ana Ochando1, Rogelio Monfort-ortiz3, Elena Sa instant download after payment.

Endometrial decidualization resistance (DR) is implicated in various gynecological and obstetric conditions. Here, using a multi-omic strategy, we unraveled the cellular and molecular characteristics of DR in patients who have sufered severe preeclampsia (sPE). Morphological analysis unveiled signifcant glandular anatomical abnormalities, confrmed histologically and quantifed by the digitization of hematoxylin and eosin-stained tissue sections. Single-cell RNA sequencing (scRNA-seq) of endometrial samples from patients with sPE (n = 11) and controls (n = 12) revealed sPE-associated shifts in cell composition, manifesting as a stromal mosaic state characterized by proliferative stromal cells (MMP11 and SFRP4) alongside IGFBP1+ decidualized cells, with concurrent epithelial mosaicism and a dearth of epithelial–stromal transition associated with decidualization. Cell–cell communication network mapping underscored aberrant crosstalk among specifc cell types, implicating crucial pathways such as endoglin, WNT and SPP1. Spatial transcriptomics in a replication cohort validated DR-associated features. Laser capture microdissection/mass spectrometry in a second replication cohort corroborated several scRNA-seq fndings, notably the absence of stromal to epithelial transition at a pathway level, indicating a disrupted response to steroid hormones, particularly estrogens. These insights shed light on potential molecular mechanisms underpinning DR pathogenesis in the context of sPE.