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Nitric Oxide Protocols Methods in Molecular Biology Vol 279 2nd Edition by Aviv Hassid ISBN 1588292371 978-1588292377

  • SKU: BELL-2185064
Nitric Oxide Protocols Methods in Molecular Biology Vol 279 2nd Edition by Aviv Hassid ISBN 1588292371 978-1588292377
$ 31.00 $ 45.00 (-31%)

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Nitric Oxide Protocols Methods in Molecular Biology Vol 279 2nd Edition by Aviv Hassid ISBN 1588292371 978-1588292377 instant download after payment.

Publisher: Humana Press
File Extension: PDF
File size: 7.42 MB
Pages: 260
Author: Aviv Hassid
ISBN: 9781588292377, 9781592598076
Language: English
Year: 2004
Edition: 2

Product desciption

Nitric Oxide Protocols Methods in Molecular Biology Vol 279 2nd Edition by Aviv Hassid ISBN 1588292371 978-1588292377 by Aviv Hassid 9781588292377, 9781592598076 instant download after payment.

Nitric Oxide Protocols Methods in Molecular Biology Vol 279 2nd Edition by Aviv Hassid - Ebook PDF Instant Download/Delivery: 1588292371, 978-1588292377

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Product details:

ISBN 10: 1588292371

ISBN 13: 978-1588292377 

Author: Aviv Hassid

It has been more than five years since the appearance of the first edition of Nitric Oxide Protocols. According to Medline, more than 32,500 publications dealing with nitric oxide have appeared in the primary literature in the int- vening time from 1999 to April 2004. These numbers attest to the continuing vitality of a field dealing with a deceptively simple, although extremely int- esting and promiscuous molecule, nitric oxide. Despite its apparent chemical simplicity, nitric oxide continues to amaze, with an extremely wide spectrum of functions and effects. In particular, the last few years have witnessed an explosion of nonclassical effects, involving the direct interaction of nitric - ide and related oxidized products with proteins to yield such novel combi- tions as nitrosylated and nitrated proteins. Another emergent area has been that of NO’s clinical applications, including interesting developments related to the virus-mediated expression of nitric oxide synthase and the use of nitric oxide in gene therapy. In Nitric Oxide Protocols, I have gathered chapters dealing with the some of the latest methodologies for the measurement of nitric oxide. Many of these deal with quantitation in biological fluids or tissues, and they cover a wide spectrum of technologies, including capillary electrophoresis, microcoaxial electrodes, the in vivo measurement of nitric oxide in exhaled air, confocal microscopy, gas chromatography–mass spectroscopy, in situ hybridization, and real-time polymerase chain reaction.

Table of contents:

  1. Front Matter

  2. Measurement of Nitric Oxide by Reconversion of Nitrate/Nitrite to NO

  3. High-Resolution Capillary Electrophoresis of Nitrite and Nitrate in Biological Samples

  4. Nitrite and Nitrate Measurements in Human Urine by Capillary Electrophoresis

  5. In Vivo Nitric Oxide Measurements Using a Microcoaxial Electrode

  6. Measurement of Exhaled Nitric Oxide

  7. Nitric Oxide Imaging in Neurons Using Confocal Microscopy

  8. Measurement of Nitric Oxide Synthase Activity In Vivo and In Vitro by Gas Chromatography-Mass Spectrometry

  9. A Nonradioactive Assay for Nitric Oxide Synthase Activity in Tissue Extracts

  10. Measurement of Nitric Oxide-Related Enzymes in the Brain by In Situ Hybridization

  11. Real-Time Polymerase Chain Reaction to Quantify mRNA for Endothelial Nitric Oxide Synthase

  12. Dioxygen-Dependent Metabolism of Nitric Oxide

  13. Proteomic Method for Identification of Tyrosine-Nitrated Proteins

  14. Immunohistochemical Detection of S-Nitrosylated Proteins

  15. Gene Therapy Techniques for the Delivery of Endothelial Nitric Oxide Synthase to the Corpora Cavernosa for Erectile Dysfunction

  16. Adenovirus-Mediated Nitric Oxide Synthase Gene Transfer Into the Nucleus Tractus Solitarius in Conscious Rats

  17. Gene Therapy in Cancer Via Use of a Retrovector Having a Tumor Specificity and Expressing Inducible Nitric Oxide Synthase

  18. Gene Transfer of Nitric Oxide Synthase Via the Use of Adeno-Associated Virus Vectors

  19. Adenovirus-Mediated Nitric Oxide Synthase Gene Transfer

  20. Back Matter

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Tags: Aviv Hassid, Nitric Oxide, Protocols, Methods, Molecular Biology, Vol 279

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