Nnosexpressing Neurons In The Mpfc Mediate Depressionrelated Behaviors In Mice Through Ppvtmpfc Projections Haiying Liang Zhibin Zhao Zhen Liu James Samsom Kaihong Chen Ying Chen Liling Chen Chengyun Cai Fang Liu by Hai-ying Liang & Zhi-bin Zhao & Zhen Liu & James Samsom & Kai-hong Chen & Ying Chen & Li-ling Chen & Cheng-yun Cai & Fang Liu instant download after payment.

Depression is one of the most common mental disorders, but its etiology remains poorly understood. Neuronal nitric oxidesynthase (nNOS) has been implicated in depression, but the role of nNOS-expressing neurons is still unknown. We usedchemogenetic strategies to show that nNOS-expressing neurons in the medial prefrontal cortex (mPFC) are essential for depressionrelated behaviors. Using electrophysiology, we determined that mPFC nNOS-expressing neurons receive projections from theposterior subregion of the paraventricular thalamic nucleus (pPVT). We show that excitatory projections from the pPVT onto mPFCnNOS-expressing neurons regulate depression-related behaviors. We further explore a mechanism in which activation of mPFCnNOS-expressing neurons leads to the subsequent release of nitric oxide (NO), which enhances the nitrosylation of cyclin1234567890();,:dependent kinase 5 (CDK5). Our data suggest a mechanism for depression involving excitatory pPVT projections onto nNOSexpressing neurons in the mPFC that represents a potential target for future treatments.Neuropsychopharmacology;