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Plant Reverse Genetics Methods and Protocols Kindle Edition by Andy Pereira ISBN 1607616823 9781607616825

  • SKU: BELL-2008984
Plant Reverse Genetics Methods and Protocols Kindle Edition by Andy Pereira ISBN 1607616823 9781607616825
$ 31.00 $ 45.00 (-31%)

4.0

96 reviews

Plant Reverse Genetics Methods and Protocols Kindle Edition by Andy Pereira ISBN 1607616823 9781607616825 instant download after payment.

Publisher: Humana Press
File Extension: PDF
File size: 6.79 MB
Pages: 285
Author: Andy Pereira
ISBN: 9781607616818, 1607616815
Language: English
Year: 2010
Edition: 1st Edition.

Product desciption

Plant Reverse Genetics Methods and Protocols Kindle Edition by Andy Pereira ISBN 1607616823 9781607616825 by Andy Pereira 9781607616818, 1607616815 instant download after payment.

Plant Reverse Genetics Methods and Protocols Kindle Edition by Andy Pereira - Ebook PDF Instant Download/Delivery: 1607616823, 9781607616825

Full download Plant Reverse Genetics Methods and Protocols Kindle Edition after payment

 

Product details:

ISBN 10: 1607616823

ISBN 13: 9781607616825 

Author: Andy Pereira

After the generation of genome sequence data from a wide variety of plants, databases are filled with sequence information of genes with no known biological function, and while bioinformatics tools can help analyze genome sequences and predict gene structures, experimental approaches to discover gene functions need to be widely implemented. In Plant Reverse Genetics: Methods and Protocols, leading researchers in the field describe cutting-edge methods, both high-throughput and genome-wide, involving the models Arabidopsis and rice as well as several other plants to provide comparative functional genomics information. With chapters on the analysis of high-throughput genome sequence data, the identification of non-coding RNA from sequence information, the comprehensive analysis of gene expression by microarrays, and metabolomic analysis, the thorough methods of the book are fully supported by scripts to aid their computational use. Written in the highly successful Methods in Molecular Biology™ series format, the chapters contain introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and essential, Plant Reverse Genetics: Methods and Protocols is an ideal guide for researchers seeking an understanding of how the complex web of plant genes work together in a systems biology view.

Table of contents:

  1. Analysis of high-throughput sequencing data

  2. Identification of plant microRNAs using expressed sequence tag analysis

  3. Microarray data analysis

  4. Setting up reverse transcription quantitative-PCR experiments

  5. Virus-induced gene silencing in Nicotiana benthamiana and other plant species

  6. Agroinoculation and agroinfiltration: Simple tools for complex gene function analyses

  7. Full-length cDNA overexpressor gene hunting system (FOX hunting system)

  8. Activation tagging with En/Spm-i/dSpm transposons in Arabidopsis

  9. Activation tagging and insertional mutagenesis in barley

  10. Methods for rice phenomics studies

  11. Development of an efficient inverse PCR method for isolating gene tags from T-DNA insertional mutants in rice

  12. Transposon insertional mutagenesis in rice

  13. Reverse genetics in Medicago truncatula using Tnt1 insertion mutants

  14. Screening Arabidopsis genotypes for drought stress resistance

  15. Protein tagging for chromatin immunoprecipitation from Arabidopsis

  16. Yeast one-hybrid screens for detection of transcription factor DNA interactions

  17. Plant metabolomics by GC-MS and differential analysis

  18. Gramene database: A hub for comparative plant genomics

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Tags: Andy Pereira, Plant, Reverse Genetics, Methods, Protocols

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