Protein Interactions Biophysical Approaches for the Study of Complex Reversible Systems 1st Edition by Peter Schuck ISBN 0387359656 9780387359656 by Peter Schuck (editor), M. Zouhair Atassi (series Editor) 0387359656, 9780387359656 instant download after payment.

Protein Interactions Biophysical Approaches for the Study of Complex Reversible Systems 1st Edition by Peter Schuck - Ebook PDF Instant Download/Delivery: 0387359656, 9780387359656
Full download Protein Interactions Biophysical Approaches for the Study of Complex Reversible Systems 1st Edition after payment

Product details:

ISBN 10: 0387359656 
ISBN 13: 9780387359656
Author: Peter Schuck

When I was invited to edit this volume, I wanted to take the opportunity to assemble reviews of different biophysical methodologies for protein interactions at a level suf?ciently detailed to understand how complex systems can be studied. There are several excellent introductory texts for biophysical methodologies, many with hands-on descriptions or embedded in general introductions to physical b- chemistry. The goal of the present volume was to present state-of-the-art reviews that do not necessarily enable the reader to carry out these techniques, but to gain a deep understanding of the biophysical observables, to stimulate creative thought on how the techniques may be applied to study a particular biological system, and to foster collaboration and multidisciplinary work. Reversible protein interactions involve noncovalent chemical bonds, pro- cing protein complexes with free energies not far from the order of magnitude of the thermal energy kT. As a consequence, they can be highly dynamic and may be controlled, for example, by protein expression levels and changes in the intracel- lar or microenvironment. Reversible protein complexes may have suf?cient stab- ity to be puri?ed for study, but frequently their short lifetime essentially limits their existence to solutions of mixtures of the binding partners in which they remain populated through dissociation and reassociation processes. To understand the function of such protein complexes, it is important to study their structure and dynamics.

Protein Interactions Biophysical Approaches for the Study of Complex Reversible Systems 1st Table of contents:

1. The Characterization of Biomolecular Interactions Using Fluorescence Fluctuation Techniques

2. Characterization of Protein–Protein Interactions Using Atomic Force Microscopy

3. Combined Solid-Phase Detection Techniques for Dissecting Multiprotein Interactions on Membranes

4. Surface Plasmon Resonance Biosensing in the Study of Ternary Systems of Interacting Proteins

5. Mass Spectrometry for Studying Protein Modifications and for Discovery of Protein Interactions

6. H/2H Exchange Mass Spectrometry of Protein Complexes

7. Elucidation of Protein–Protein and Protein–Ligand Interactions by NMR Spectroscopy

8. Application of Isothermal Titration Calorimetry in Exploring the Extended Interface

9. Solvent Mediated Protein–Protein Interactions

10. Sedimentation Equilibrium Analytical Ultracentrifugation for Multicomponent Protein Interactions

11. Structure Analysis of Macromolecular Complexes by Solution Small-Angle Scattering

12. Fluorescence Detection of Proximity

13. Steady-State and Time-Resolved Emission Anisotropy

14. Analysis of Protein-DNA Equilibria by Native Gel Electrophoresis

15. Electrospray Ionization Mass Spectrometry and the Study of Protein Complexes

16. Sedimentation Velocity in the Study of Reversible Multiprotein Complexes

People also search for Protein Interactions Biophysical Approaches for the Study of Complex Reversible Systems 1st:

protein protein interactions
    
rna protein interactions
    
dna protein interactions
    
what factors contribute to protein protein interactions
    
string protein interactions
    
protein protein interactions database

Tags: Peter Schuck, Interactions, Biophysical