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Recombinant Protein Expression In Mammalian Cells Methods And Protocols 1st Ed David L Hacker

  • SKU: BELL-7319684
Recombinant Protein Expression In Mammalian Cells Methods And Protocols 1st Ed David L Hacker
$ 31.00 $ 45.00 (-31%)

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Recombinant Protein Expression In Mammalian Cells Methods And Protocols 1st Ed David L Hacker instant download after payment.

Publisher: Springer New York;Humana Press
File Extension: PDF
File size: 8.31 MB
Author: David L. Hacker
ISBN: 9781493987290, 9781493987306, 1493987291, 1493987305
Language: English
Year: 2018
Edition: 1st ed.

Product desciption

Recombinant Protein Expression In Mammalian Cells Methods And Protocols 1st Ed David L Hacker by David L. Hacker 9781493987290, 9781493987306, 1493987291, 1493987305 instant download after payment.

This detailed volume explores advances in vector design, DNA delivery, cell cultivation, host cell engineering, and bioprocess optimization within the study of recombinant protein expression in mammalian cells. The majority of the protocols employ either Chinese hamster ovary cells (CHO) or human embryonic kidney 293 cells (HEK293), the workhorses of the field, as the production host; however, the methods can be adapted to other mammalian hosts under the appropriate cell-specific conditions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and convenient, Recombinant Protein Expression in Mammalian Cells: Methods and Protocols aims to aid researchers in building on our knowledge of protein structure and function and to speed the discovery of new therapeutic proteins.

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