Replacing Animal Models A Practical Guide To Creating And Using Culturebased Biomimetic Alternatives Jamie Davies by Jamie Davies 9780470974254, 9781119940685, 0470974257, 1119940680 instant download after payment.

The book is neither political nor polemical: it is technical, illustrating by example how alternatives can be developed and used and providing useful advice on developing others. After looking at the reasons for and potential benefits of alternatives to animal experiments, the book covers a range of methods and examples emphasising the design considerations that went into each system. The chapters also include 'case studies' that illustrate the ways in which culture models can be used to answer a range of important biological questions of direct relevance to human development, physiology, disease and healing.

The thesis of this book is not that all animal experimentation can be replaced, now or in the near future, by equally effective or superior alternatives. Rather, the premise is that there is substantial opportunity, here and now, to do some common types of experiment better in vitro than in vivo, and that doing so will result in both scientific and ethical gains.Content:
Chapter 1 Potential Advantages of Using Biomimetic Alternatives (pages 1–11): Jamie Davies
Chapter 2 Overview of Biomimetic Alternatives (pages 13–19): Jamie Davies
Chapter 3 Pancreatic Islets (pages 21–33): Eli C Lewis
Chapter 4 Endometrial Organoid Culture (pages 35–44): Merja Blauer
Chapter 5 Modelling Lymphatic and Blood Capillary Patterning (pages 45–56): Francoise Bruyere, Catherine Maillard, Charlotte Erpicum and Agnes Noel
Chapter 6 Precision?Cut Lung Slices (PCLS) (pages 57–67): Christian Martin and Stefan Uhlig
Chapter 7 Human Colon Tissue in Organ Culture (pages 69–80): Michael K Dame and James Varani
Chapter 8 Fetal Organ Culture (pages 81–87): Jamie Davies
Chapter 9 Design of a Mechanical Loading Device to Culture Intact Bovine Spinal Motion Segments under Multiaxial Motion (pages 89–105): Jochen Walser, Stephen John Ferguson and Benjamin Gantenbein?Ritter
Chapter 10 Magnetic Assembly of Tissue Surrogates (pages 107–114): Chien?Yu Fu and Hwan?You Chang
Chapter 11 Assembly of Renal Tissues by Cellular Self?Organization (pages 115–122): Mathieu Unbekandt
Chapter 12 Hierarchical Screening of Pathways: Using Cell and Organ Cultures to Reduce use of Transgenic Mice (pages 123–136): Guangping Tai and Jamie Davies
Chapter 13 Lung Organoid Culture to Study Responses to Viruses (pages 137–149): Wenxin Wu, J Leland Booth and Jordan P Metcalf
Chapter 14 Organ?Cultured Human Skin for the Study of Epithelial Cell Invasion of Stroma (pages 151–158): James Varani
Chapter 15 Organotypic Mandibular Cultures for the Study of Inflammatory Bone Pathology (pages 159–166): Alastair J Sloan, Sarah Y Taylor and Emma L Smith
Chapter 16 Three?Dimensional, High?Density and Tissue Engineered Culture Models of Articular Cartilage (pages 167–192): Ali Mobasheri, Sara Kelly, Abigail L. Clutterbuck, Constanze Buhrmann and Mehdi Shakibaei
Chapter 17 Concluding Remarks (pages 193–194): Jamie Davies