Rna Purification And Analysis Sample Preparation Extraction Chromatography Dr Douglas T Gjerde by Dr. Douglas T. Gjerde, Dr. Lee Hoang, Dr. David Hornby(auth.) 9783527321162, 9783527627196, 3527321160, 3527627197 instant download after payment.

This first book on the market covers the many new and important RNA species discovered over the past five years, explaining current methods for the enrichment, separation and purification of these novel RNAs.
Building up from general principles of RNA biochemistry and biophysics, this book addresses the practical aspects relevant to the laboratory researcher throughout, while discussing the performance and potential problems of the methods discussed. An appendix contains a glossary with the important terms and techniques used in RNA analysis.
By explaining the basic and working principles of the methods, the book allows biochemists and molecular biologists to gain much more expertise than by simply repeating a pre-formulated protocol, enabling them to select the procedure and materials best suited to the RNA analysis task at hand. As a result, they will be able to develop new protocols where needed and optimize and fine-tune the general purpose standard protocols that come with the purification equipment and instrumentation.Content:
Chapter 1 RNA Extraction, Separation, and Analysis (pages 1–16):
Chapter 2 Biological and Chemical RNA (pages 17–35):
Chapter 3 RNA Separation: Substrates, Functional Groups, Mechanisms, and Control (pages 37–66):
Chapter 4 RNA Extraction and Analysis (pages 67–80):
Chapter 5 RNA Chromatography (pages 81–99):
Chapter 6 RNA Chromatography Separation and Analysis (pages 101–125):
Chapter 7 RNA Structure–Function Probing (pages 127–145):