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Test No 442e In Vitro Skin Sensitisation Human Cell Line Activation Test Hclat Coll

Name: Test No 442e In Vitro Skin Sensitisation Human Cell Line Activation Test Hclat Coll
SKU: BELL-7238692
Price: 31.00 USD
Availability: InStock
Rating: 4.8 (54 reviews)

SKU: BELL-7238692

$ 31.00 ~~$ 45.00~~ (-31%)

4.8

54 reviews

Test No 442e In Vitro Skin Sensitisation Human Cell Line Activation Test Hclat Coll instant download after payment.

Publisher: OECD Publishing

File Extension: PDF

File size: 1.31 MB

Pages: 21

Author: coll.

ISBN: 9789264264359, 9264264353

Language: English

Year: 2016

Product desciption

Test No 442e In Vitro Skin Sensitisation Human Cell Line Activation Test Hclat Coll by Coll. 9789264264359, 9264264353 instant download after payment.

The present Test Guideline addresses the human health hazard endpoint skin sensitisation, following exposure to a test chemical. Skin sensitisation refers to an allergic response following skin contact with the tested chemical, as defined by the United Nations Globally Harmonized System of Classification and Labelling of Chemicals (UN GHS). This Test Guideline (TG) provides an in vitro procedure (the human cell Line Activation Test h-CLAT method) used for supporting the discrimination between skin sensitisers and non-sensitisers in accordance with the UN GHS. The h-CLAT method is proposed to address the third key event of the skin sensitisation AOP by quantifying changes in the expression of cell surface markers associated with the process of activation of monocytes and dendritic cells (DC) (i.e. CD86 and CD54), in the human monocytic leukaemia cell line THP-1, following exposure to sensitising test chemical. These surface molecules are typical markers of monocytic THP-1 activation and may mimic DC activation, which plays a critical role in T-cell priming. The changes of surface marker expression are measured by flow cytometry following cell staining with fluorochrome-tagged antibodies. The relative fluorescence intensity of surface markers compared to solvent/vehicle control are calculated and used in the prediction model, to support the discrimination between sensitisers and non-sensitisers.