Dna Topoisomerase Protocols Volume 1 Dna Topology And Enzymes Methods In Molecular Biology Vol 94 Bjornsti by Bjornsti, Mary-ann; Osheroff, Neil 9780585243993, 9780896034440, 0585243999, 0896034445 instant download after payment.

DNA Topoisomerase Protocols is an unprecedented collection of cutting-edge experimental protocols for investigating DNA structure, topology, and DNA topoisomerase function. Described by expert experimentalists who have perfected the techniques, these time-proven methods utilize new approaches to study changes in DNA topology (linking number, knotting, catenation, and relaxation) and DNA structure (bending), as well as to assess chromosome structure through FLP-mediated recombination and to analyze bacterial nucleoid structure. State-of-the-art protocols also detail the expression and purification of bacterial, viral, and eukaryotic DNA topoisomerases from a variety of sources, including baculovirus, bacterial, yeast, and mammalian expression systems.