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ISBN 10: 0471899194
ISBN 13: 978-0471899198
Author: Corinne A. Michels
Molecular Genetic Analysis is an advanced textbook to teach the theory and practice of molecular genetic analysis to senior undergraduates and graduates studying genetics, molecular biology and cell biology.
This book uses a case study approach, with the yeast Saccharomyces as the model genetic organism, to explain the theory and practice of molecular genetic analysis. It provides enough information so readers will be able to apply the approach to their own research project.
Introduction
Section I: Saccharomyces cerevisiae as a Genetic Research Organism
Saccharomyces cerevisiae as a Genetic Model Organism
Techniques in Cell and Molecular Biology
Saccharomyces Cell Structure
Section II: Techniques of Genetic Analysis
Mutant Hunts – To Select or to Screen (perhaps even by brute force)
Complementation Analysis: How Many Genes Are Involved?
Epistasis Analysis
Gene Isolation and Analysis of Multiple Mutant Alleles
Suppression Analysis
Enhancement and Synthetic Phenotypes
Two-hybrid Analysis
Advanced Concepts in Molecular Genetic Analysis
Genomic Analysis
Section III: Case Studies from the Saccharomyces Genetic Literature
Case Study I: Glucose Sensing and Signaling Mechanisms in Saccharomyces
Case Study II: Secretion, Exocytosis and Vesicle Trafficking in Saccharomyces
Case Study III: The Cell Division Cycle of Saccharomyces
Case Study IV: Mating-type Pheromone Response Pathway of Saccharomyces
Index
genetic techniques for biological research
genetic techniques
genetic research methods
scientific technique related to genetics
biology research techniques
techniques for genetic engineering
Tags: Corinne A Michels, Genetic Techniques, Biological Research, Case Study Approach