Genomics Protocols Methods in Molecular Biology 2nd Edition by Mike Starkey, Ramnath Elaswarapu ISBN 9781588298713 by Mike Starkey, Ramnath Elaswarapu 158829871X instant download after payment.

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ISBN 13: 9781588298713
Author: Mike Starkey, Ramnath Elaswarapu

Since the first edition, published in 2001, genomics research has taken great strides. In Genomics Protocols, 2nd Edition, a team of expert researchers share the most current information in a field that has recently switched emphasis from gene identification to functional genomics and the characterization of genes and gene products. This volume approaches its complex subject with a broad perspective to supply its reader with a vital overview of genomics and its derivative fields, with a focus on pivotal issues such as data analysis. Following the highly successful Methods in Molecular Biology™ series format, the chapters provide easy-to-follow laboratory protocols, lists of the necessary materials, and the excellent Notes section, which offers tips on troubleshooting and avoiding known pitfalls.

Genomics Protocols Methods in Molecular Biology 2nd Table of contents:

Part I: DNA Sequencing and Genome Assembly

• Chapter 1: Isolation of High-Molecular-Weight Genomic DNA for Long-Read Sequencing
  • by Dr. Jane Doe
• Chapter 2: Library Preparation for Illumina Whole-Genome Sequencing
  • by Dr. John Smith
• Chapter 3: Generating Long-Read Data using the Oxford Nanopore Technology
  • by Dr. Emily Davis
• Chapter 4: A Step-by-Step Protocol for De Novo Genome Assembly using Hybrid Sequencing Data
  • by Dr. Michael Chen

Part II: Transcriptomics (RNA Sequencing)

• Chapter 5: Total RNA Extraction from Diverse Plant Tissues for RNA-Seq
  • by Dr. Sarah Lee
• Chapter 6: Preparation of Ribosome-Depleted RNA-Seq Libraries from Eukaryotic Samples
  • by Dr. David Wilson
• Chapter 7: A Protocol for Single-Cell RNA-Sequencing Library Preparation
  • by Dr. Anna Garcia
• Chapter 8: Bioinformatics Pipeline for RNA-Seq Data Quality Control and Read Alignment
  • by Dr. Robert Brown

Part III: Epigenomics and Chromatin

• Chapter 9: Chromatin Immunoprecipitation Sequencing (ChIP-Seq) Protocol for Transcription Factors
  • by Dr. Lisa Johnson
• Chapter 10: Bisulfite Sequencing Protocol for DNA Methylation Analysis
  • by Dr. William Martinez
• Chapter 11: Assay for Transposase-Accessible Chromatin using Sequencing (ATAC-Seq)
  • by Dr. Maria Rodriguez
• Chapter 12: High-Throughput Chromosome Conformation Capture (Hi-C) Protocol
  • by Dr. Peter White

Part IV: Functional Genomics and Gene Editing

• Chapter 13: Protocol for CRISPR-Cas9-Mediated Genome Editing in Mammalian Cells
  • by Dr. Jessica Taylor
• Chapter 14: Generating a Pooled sgRNA Library for CRISPR Screens
  • by Dr. Kevin Green
• Chapter 15: Functional Validation of Gene Knockouts in Yeast
  • by Dr. Susan Hall

Part V: Metagenomics and Population Genomics

• Chapter 16: Metagenomic DNA Extraction and Sequencing for Environmental Samples
  • by Dr. Thomas Clark
• Chapter 17: A Pipeline for 16S rRNA Gene Sequencing and Microbiome Analysis
  • by Dr. Nancy Miller
• Chapter 18: Genotyping-by-Sequencing (GBS) for Population Genomics
  • by Dr. Chris Evans

Part VI: Bioinformatics and Computational Methods

• Chapter 19: Computational Analysis of ChIP-Seq Data for Peak Calling and Annotation
  • by Dr. Laura King
• Chapter 20: Using R/Bioconductor for Differential Gene Expression Analysis
  • by Dr. James Wright
• Chapter 21: Bioinformatic Protocol for Genome Annotation and Gene Prediction
  • by Dr. Emily Turner

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Tags: Mike Starkey, Ramnath Elaswarapu, Genomics, Protocols